Xanthophylls of the major photosynthetic light-harvesting complex of plants: identification, conformation and dynamics.

摘要: Abstract The electronic transitions of lutein and neoxanthin in the major light-harvesting complex, LHCIIb, have been identified for first time. It was found that 0–0, 0–1 0–2 were located around 486, 457 430 nm, whilst those dependent on oligomerisation state. For monomer, absorption bands at 495, 466 437 nm. Trimerisation caused a decrease parallel appearance an additional band 510 which by resonance Raman excitation spectra to originate from lutein. Circular dichroism measurements together with analysis ν4 region xanthophylls suggested this molecule is distorted trimer. This feature not predicted LHCIIb atomic model Kuhlbrandt co-workers [Kuhlbrandt, W., Wang, D.N. Fugiyoshi, Y. (1994) Nature 367, 614–621] important step understanding pigment dynamics complex. Oligomerisation trimers led specific distortion molecule. These observations suggest sense protein conformation may reflect their special role assembly function antenna higher plants.