Influence of detergent concentration on aggregation and spectroscopic properties of light-harvesting complex II
作者： Bernd Voigt , Maria Krikunova , Heiko Lokstein
DOI: 10.1007/S11120-007-9250-5
关键词:
摘要: Aggregation of photosynthetic light-harvesting complexes strongly influences their spectroscopic properties. Fluorescence yield and excited state lifetimes the main complex (LHC II) higher plants depend on its aggregation state. Detergents are commonly used to solubilize membrane proteins and/or circumvent in aqueous environments. Nonlinear polarization spectroscopy frequency domain (NLPF) was performed with LHC II over a wide concentration range mild detergent n-dodecyl β-d-maltoside (β-DM). Additionally, conventional absorption-, fluorescence- circular dichroism-spectra were measured.
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