The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate)
作者： Luccas Missfeldt Sanches , Denise Freitas Siqueira Petri , Letícia Dias Melo Carrasco , Ana Maria Carmona-Ribeiro , None
DOI: 10.1186/S12951-015-0123-3
关键词: Antimicrobial polymer 、 Polymer chemistry 、 Cationic polymerization 、 Emulsion polymerization 、 Antimicrobial 、 Dispersity 、 Allyl compound 、 Chemistry 、 Drug carrier 、 Nanoparticle
摘要: Several cationic polymers exhibit a useful antimicrobial property, however the structure–activity relationship still requires more complete investigation. The main objective of this work is comparison between activity and toxicity free immobilized poly (diallyldimethylammonium) chloride (PDDA) in biocompatible (methylmethacrylate) (PMMA) nanoparticles (NPs). NPs synthesis by emulsion polymerization performed over range [PDDA] at two methylmethacrylate (MMA) concentrations. PMMA/PDDA dispersions are characterized dynamic light-scattering for sizing, polydispersity zeta-potential analysis, scanning electron microscopy (SEM), plating plus colony forming unities (CFU) counting determination minimal microbicidal concentrations (MMC) against Escherichia coli, Staphylococcus aureus Candida albicans hemolysis evaluation mammalian erythrocytes. There high colloidal stability [PDDA]. diverse microorganisms reduces cell viability eight-logs (E. coli), seven-logs (S. aureus) or two-logs (C. albicans). completely kill E. coli that innocuous to Free PDDA higher than one observed NPs. no induced-hemolysis MMC contrast hemolytic effect Hemolysis 15 % S. C. albicans. mobility polymer determines its access inner layers wall membrane, major sites action. freedom does matter determining low absence hemolysis.
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fapesp.br参考文章(43)
Scientific Methods for the Study of Polymer Colloids and Their Applications Kluwer Academic Publishers. ,(1990) , 10.1007/978-94-009-1950-1
Pascale Buchert, Françoise Candau, Polymerization in microemulsions: I. Formulation and structural properties of microemulsions containing a cationic monomer joint international conference on information sciences. ,vol. 136, pp. 527- 540 ,(1990) , 10.1016/0021-9797(90)90399-9
Irma A.J.M. Bakker-Woudenberg, Raymond M. Schiffelers, Gert Storm, Martin J. Becker, Luke Guo, Long-Circulating Sterically Stabilized Liposomes in the Treatment of Infections Methods in Enzymology. ,vol. 391, pp. 228- 260 ,(2005) , 10.1016/S0076-6879(05)91014-8
Marta Álvarez-Paino, Alexandra Muñoz-Bonilla, Fátima López-Fabal, José L. Gómez-Garcés, Johan P. A. Heuts, Marta Fernández-García, Functional surfaces obtained from emulsion polymerization using antimicrobial glycosylated block copolymers as surfactants Polymer Chemistry. ,vol. 6, pp. 6171- 6181 ,(2015) , 10.1039/C5PY00776C
Leticia D Melo, Renata R Palombo, Denise FS Petri, Michael Bruns, Edla MA Pereira, Ana M Carmona-Ribeiro, None, Structure-activity relationship for quaternary ammonium compounds hybridized with poly(methyl methacrylate). ACS Applied Materials & Interfaces. ,vol. 3, pp. 1933- 1939 ,(2011) , 10.1021/AM200150T
M. Firat Ilker, Klaus Nüsslein, Gregory N. Tew, E. Bryan Coughlin, Tuning the Hemolytic and Antibacterial Activities of Amphiphilic Polynorbornene Derivatives Journal of the American Chemical Society. ,vol. 126, pp. 15870- 15875 ,(2004) , 10.1021/JA045664D
Markus Antonietti, Sabine Lohmann, Cornelius Van Niel, Polymerization in microemulsion. 2. Surface control and functionalization of microparticles Macromolecules. ,vol. 25, pp. 1139- 1143 ,(1992) , 10.1021/MA00029A021
Satoshi Imazato, Ji-hua Chen, Sai Ma, Naomi Izutani, Fang Li, Antibacterial resin monomers based on quaternary ammonium and their benefits in restorative dentistry Japanese Dental Science Review. ,vol. 48, pp. 115- 125 ,(2012) , 10.1016/J.JDSR.2012.02.003
Benjamin D. Brooks, Amanda E. Brooks, Therapeutic strategies to combat antibiotic resistance. Advanced Drug Delivery Reviews. ,vol. 78, pp. 14- 27 ,(2014) , 10.1016/J.ADDR.2014.10.027
Edla M. A. Pereira, Priscila M. Kosaka, Heloísa Rosa, Débora B. Vieira, Yoshio Kawano, Denise F. S. Petri, Ana M. Carmona-Ribeiro, Hybrid materials from intermolecular associations between cationic lipid and polymers. Journal of Physical Chemistry B. ,vol. 112, pp. 9301- 9310 ,(2008) , 10.1021/JP801297T