Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines

摘要: The methylated DNA immunoprecipitation microarray (MeDIP-chip) is a genome-wide, high-resolution approach to detect methylation in whole genome or CpG (cytosine base followed by guanine base) islands. method utilizes anti-methylcytosine antibody immunoprecipitate that contains highly sites. Enriched can be interrogated using microarrays massive parallel sequencing techniques. This combined allows researchers rapidly identify regions genome-wide manner, and compare patterns between two samples with diversely different status. MeDIP-chip has been applied successfully for analyses of the targets including animal plant tissues. Here we present protocol routinely used our laboratory, illustrated specific examples from analysis breast cancer cell lines. Potential technical pitfalls solutions are also provided serve as workflow guidelines.