Reconstitution of the vascular wall in vitro. A novel model to study interactions between endothelial and smooth muscle cells.

摘要: Abstract To study the biology of endothelium under conditions that mimic architecture vessel wall, endothelial cells were grown on a collagen lattice containing multilayer smooth muscle cells. Light and electron microscopy such cultures revealed confluent monolayer flattened In co-culture, tend to elongate, whereas in absence cells, show polygonal morphology typical for polystyrene substrates. As conditioned culture media contain substances may both promote or inhibit growth availability this wall model prompted us examine what extent regulate proliferation when these are maintained co-culture. Here we suppress co-existing This finding suggests physiological balance action growth-promoting growth-inhibiting produced by is favour latter.