A hyperbranched β-d-glucan with compact coil conformation from Lignosus rhinocerotis sclerotia.

摘要: Abstract An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC–MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation methylation analysis. The LRP had a (1 → 3)-β- d -Glcp backbone every three residues bearing (1 → 6)-linked hyperbranched side chain that contained (1 → 6)-β- as secondary main two terminal β- linked at O3. degree of branching 0.76 GC–MS analysis, implying highly branched structure for LRP. Mw, z1/2, Rh [η] values in 0.25 M LiCl/DMSO measured SEC-MALLS-Vis-RI combination technology to be 2.88 × 105 g/mol, 30.36 nm, 22.34 nm 131.50 ml/g, respectively. Furthermore, the exponent α [η]–Mw, β z1/2–Mw, fractal dimension df molecular parameter ρ 0.20, 0.33, 2.50 1.36, demonstrating adopted compact coil conformation LiCl/DMSO.