Selection of Lactobacillus acidophilus strains for use in "acidophilus products".

作者: M. C. Johnson , Bibek Ray , Tarun Bhowmik

DOI: 10.1007/BF00393929

关键词: MicrobiologyLactobacillus crispatusBiologyBacteriaLactobacillus acidophilusLactoseLysozymeFermentationProtein subunitLactobacillus gasseri

摘要: Recent studies by DNA-DNA hybridization revealed that strains now designated as L. acidophilus, can be divided into several groups and only one group should classified acidophilus. We studied phenotypic characteristics in representative from the six DNA-homology of No specific pattern was observed among for fermentation eight carbohydrates, growth at 15 45 degrees C, resistance to 0.2% oxgall, lysis lysozyme or sensitivity 17 antibiotics. However, some differences were beta-galactosidase (beta-gal) activity surface layer (s-layer) protein. Strains B1 do not have a s-layer beta-gal while B2 also lack but possess beta-gal. All A1, A2, A3 A4, capable growing lactose, composed protein subunits different molecular weights (MW). A1 homology with 46 Kd other A varied MW within each group. On basis these two traits isolates unknown been tentatively placed groups. acidophilus showed strain variation rate acid production growth. For use dietary adjuncts, selected three desirable traits. They maintained grown media containing lactose.

参考文章(23)
Eckhard Lauer, Christine Helming, Otto Kandler, Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Moquot as revealed by biochemical characteristics and DNA-DNA hybridisation. Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ökologische Mikrobiologie. ,vol. 1, pp. 150- 168 ,(1980) , 10.1016/S0172-5564(80)80037-6
Eckhard Lauer, Otto Kandler, Lactobacillus gasseri sp. nov., a new species of the subgenus Thermobacterium. Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ökologische Mikrobiologie. ,vol. 1, pp. 75- 78 ,(1980) , 10.1016/S0172-5564(80)80019-4
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
J. E. Citti, W. E. Sandine, P. R. Elliker, β-Galactosidase of Streptococcus lactis Journal of Bacteriology. ,vol. 89, pp. 937- 942 ,(1965) , 10.1128/JB.89.4.937-942.1965
S.E. Gilliland, M.L. Speck, G.F. Nauyok, F.G. Giesbrecht, Influence of Consuming Nonfermented Milk Containing Lactobacillus acidophilus on Fecal Flora of Healthy Males Journal of Dairy Science. ,vol. 61, pp. 1- 10 ,(1978) , 10.3168/JDS.S0022-0302(78)83543-7
J. L. JOHNSON, C. F. PHELPS, C. S. CUMMINS, J. LONDON, F. GASSER, Taxonomy of the Lactobacillus acidophilus Group International Journal of Systematic and Evolutionary Microbiology. ,vol. 30, pp. 53- 68 ,(1980) , 10.1099/00207713-30-1-53
M L Clements, M M Levine, R E Black, R M Robins-Browne, L A Cisneros, G L Drusano, C F Lanata, A J Saah, Lactobacillus prophylaxis for diarrhea due to enterotoxigenic Escherichia coli. Antimicrobial Agents and Chemotherapy. ,vol. 20, pp. 104- 108 ,(1981) , 10.1128/AAC.20.1.104
Tarun Bhowmik, M.C. Johnson, Bibek Ray, Isolation and partial characterization of the surface protein of Lactobacillus acidophilus strains International Journal of Food Microbiology. ,vol. 2, pp. 311- 321 ,(1985) , 10.1016/0168-1605(85)90044-3