Variable NAT1 enzyme activity in long-term cultured human HaCaT keratinocytes.

摘要: Since animal testing should be avoided whenever possible, the development of in vitro tests for predicting effect chemicals becomes a major field. This rise test systems led to an increased requirement well-characterized continuously growing cell lines. Monitoring cells during and routine culture is necessary gain relevant reproducible results. In present study, influence passaging under constant conditions on human keratinocyte line HaCaT was investigated. Data demonstrated that growth rate rose with increasing passages. Doubling times were decreased 24 ± 0.6 h late passages (12–16), comparison 36.2 1.5 early (2–8). These data confirmed by fall mRNA expression levels keratin 1 transglutaminase within Furthermore, activities xenobiotic metabolizing phase II enzyme N-acetyltransferase (NAT1) higher compared earl...