作者: ROGER Y. TSIEN
DOI: 10.1021/CEN-V072N029.P034
关键词: Molecular probe 、 Chemistry 、 Intracellular 、 Nanotechnology 、 Biophysics 、 Cell 、 Fluorescence-lifetime imaging microscopy 、 Experimental challenge 、 Fluorescence 、 General Chemical Engineering
摘要: Living cells are intricate and versatile micromodules for processing information. They can orchestrate their responses to a wide variety of stimuli via complex fluctuations—in both space time—of internal biochemistry. To understand how they do this, one needs see where when these fluctuations occur carry The experimental challenge actually seeing has become feasible, thanks the design synthesis molecular probes that be placed within detect reversibly bind messenger molecules act as imaging agents. Fluorescent most popular, because fluorescence is usually highly specific, extremely sensitive, amenable microscopic detection. intracellular agents an area organic chemistry essential progress biology. common target Ca 2+ , this divalent cation involved in host biological responses...