Direct Visualization of Secretion from Single Bovine Adrenal Chromaffin Cells by Laser-Induced Native Fluorescence Imaging Microscopy:

摘要: Direct visualization of the secretion process individual bovine adrenal chromaffin cells was achieved with laser-induced native fluorescence imaging microscopy. By monitoring catecholamines excited by 275 nm laser line an intensified charge-coupled-device (CCD) camera, we obtained good temporal and spatial resolution simultaneously without using additional fluorescent probes. Large variations were found among in terms amounts secreted rates secretion. Different regions a cell also behave differently during process. However, degree this local heterogeneity is smaller than neurons neuralgia. The influence deep-ultraviolet (UV) excitation on discussed. This quantitative technique provides useful noninvasive approach for study dynamic cellular changes understanding molecular mechanisms secretory processes.