Direct toxic effect of the radiocontrast agent diatrizoate on renal proximal tubule cells

摘要: The pathophysiology of radiocontrast agent-induced acute renal failure is presently unclear. To test for a possible direct deleterious effect diatrizoate, commonly used agent, on tubule cells, suspensions enriched in rabbit proximal segments were incubated with sodium diatrizoate. After these manipulations, variety well-established metabolic parameters to quantitate the extent cell injury measured. Diatrizoate (25 mM) produced significant declines K+, ATP, and total adenine nucleotide (TAN) contents, decreases basal uncoupled respiratory rates, increase Ca2+ content, demonstrating development induced by These effects dose related progressive increasing incubation time from 97.5 157.5 min. N-methylglucosamine (meglumine) viability was also evaluated. Meglumine low molecular weight amino-substituted cationic compound added dye solutions. had lower K+ content decrease both rates. alterations slightly additive diatrizoate that meglumine greater tubule-metabolic compared sodium. A period 22.5 min hypoxia caused changes each quantitative indices viability, potentiated degree hypoxia-induced injury. results demonstrate directly toxic cells. Meglumine, cation containing solutions, moderate epithelial cells toxicity aggravated 22.5-min hypoxia. experiments thus provide evidence which hypoxic