Proteomic and metabolomic studies on milk during bovine mastitis

摘要: The principal objectives of the study presented in this thesis were to changes milk proteomes, peptidomes and metabolomes during course bovine mastitis comparison with normal samples discover new biomarkers using various modern up-to-date methodologies such as proteomics, peptidomics metabolomics. Bovine caused by bacterial infection mammary gland dairy cows is often associated loss production due a reduction composition quality which turns, lead negative economic impact on industry. Two important acute phase proteins (APPs) serve valuable investigated every chapter developed validated enzyme linked immunosorbent assay (ELISA) for haptoglobin commercially available ELISA serum amyloid A3 (M-SAA3). These APPs quantified alongside somatic cell counts (SCC) California Mastitis Test (CMT) confirm disease status each animal used study. Proteomic applied including 1D gel electrophoresis, 2D MALDI-TOF analysis difference electrophoresis investigate proteome both subclinical clinical mastitic healthy samples. However these investigations did not reveal novel mastitis. Next, peptidomic peptidome detect presence any significant CE-MS LC-MS/MS. A total 31 14 polypeptides can be discriminate control from infected groups E. coli S. aureus respectively. Lastly, metabolomic methodology was an intention metabolome ultimately mastitis. Di- tri-peptides found higher than based metabolic pathways, arachidonic, arginine galactose metabolites seen increased samples. Overall, findings detailed indicate that use advanced proteomic could deliver their promise discovery potential biomarkers. Further studies are needed validation proposed it hoped better prevention treatment methods achieved future.