A simple procedure for removal of triton X-100 from protein samples

作者： P.W. Holloway

DOI: 10.1016/0003-2697(73)90436-3

关键词: Adsorption 、 Copolymer 、 Triton X-100 、 Spectrophotometry 、 Chemistry 、 Dilution 、 Chromatography

摘要: … This report presents a simple, rapid, and mild procedure for removal of Triton X-100. It was found that Triton X-100 is strongly adsorbed onto Bio-Beads SM-2, a neutral porous …

参考文章(8)

Allan G. Gornall, Charles J. Bardawill, Maxima M. David, Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. ,vol. 177, pp. 751- 766 ,(1949) , 10.1016/S0021-9258(18)57021-6

S. Rottem, Olga Stein, S. Razin, Reassembly of Mycoplasma membranes disaggregated by detergents Archives of Biochemistry and Biophysics. ,vol. 125, pp. 46- 56 ,(1968) , 10.1016/0003-9861(68)90637-1

P. W. Holloway, J. T. Katz, A requirement for cytochrome b 5 in microsomal stearyl coenzyme A desaturation. Biochemistry. ,vol. 11, pp. 3689- 3696 ,(1972) , 10.1021/BI00770A005

Paul A. Loach, Diane L. Sekura, R. M. Hadsell, A. Stemer, Quantitative dissolution of the membrane and preparation of photoreceptor subunits from Rhodopseudomonas spheroides. Biochemistry. ,vol. 9, pp. 724- 733 ,(1970) , 10.1021/BI00806A004

Shmuel Razin, Reconstitution of biological membranes Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 265, pp. 241- 296 ,(1972) , 10.1016/0304-4157(72)90004-4

Kai Simons, Ari Helenius, Removal of lipids from human plasma low-density lipoprotein by detergents Biochemistry. ,vol. 10, pp. 2542- 2547 ,(1971) , 10.1021/BI00789A019

G. Popják, P. W. Holloway, R. P. M. Bond, M. Roberts, Analogues of geranyl pyrophosphate as inhibitors of prenyltransferase Biochemical Journal. ,vol. 111, pp. 333- 343 ,(1969) , 10.1042/BJ1110333

S Razin, Reconstruction of biological membranes. Biochimica et Biophysica Acta. ,vol. 265, pp. 241- 296 ,(1972)