Gingipain R1 and Lipopolysaccharide From Porphyromonas gingivalis Have Major Effects on Blood Clot Morphology and Mechanics.

摘要: Background: Porphyromonas gingivalis and its inflammagens are associated with a number of systemic diseases, such as cardiovascular disease type 2 diabetes (T2DM). The proteases, gingipains, have also recently been identified in the brains Alzheimer's patients blood Parkinson's patients. Bacterial inflammagens, including lipopolysaccharides (LPSs) various proteases circulation, may drive inflammation. Methods: Here, we investigate effects bacterial products LPS from Escherichia coli gingivalis, P. gingipain [recombinant R1 (RgpA)], on clot architecture formation whole plasma healthy individuals, well purified fibrinogen models. Structural analysis clots was performed using confocal microscopy, scanning electron AFM-Raman imaging. We use thromboelastography® (TEG®) rheometry to compare static dynamic mechanical properties clots. Results: found that these interact fibrin(ogen) this interaction causes anomalous clotting. Conclusions: These techniques, combination, provide insight into health, particularly structure mechanics.