Electrospun, Biofunctionalized Fibers as Tailored in vitro Substrates for Keratinocyte Cell Culture
作者： Dirk Grafahrend , Karl-Heinz Heffels , Martin Möller , Doris Klee , Jürgen Groll
关键词: Polymer chemistry 、 PLGA 、 Adhesion 、 HaCaT 、 Cell adhesion 、 Chemistry 、 Extracellular matrix 、 Biophysics 、 Tissue engineering 、 Nanofiber 、 Fibronectin 、 Biotechnology 、 Materials Chemistry 、 Bioengineering 、 Polymers and Plastics 、 Biomaterials
摘要: Cell adhesion preventing fiber surfaces were tailored differently with bioactive peptides (a fibronectin fragment (GRGDS), a collagen IV (GEFYFDLRLKGDK) and combination of both) to provide an artificial extracellular matrix as substrate for HaCaT keratinocyte cell culture. Therefore, polymer blend containing six-arm star-shaped statistical copolymer ethylene oxide propylene in the ratio 80:20 (NCO-sP[EO-co-PO]) poly-[D,L-(lactide-co-glycolide)] (PLGA) was electrospun. The resulting fibers biofunctionalized investigated vitro substrates using kerationcyte line. Appropriate surface chemistry on these electrospun proved prevent keratinocytes, while additional immobilization certain peptide sequences induced adhesion. These specific enable investigation immobilized active molecules subsequent cellular response scaffold. keratinocytes found selectively adhere those modified either segment GEFYFDLRLKGDK or mixture two GRGDS (1:1). However, synergistic effects both (the fragment) seem significantly increase numbers adherent keratinocytes.
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