Expression and Putative Function of Innate Immunity Genes under In Situ Conditions in the Symbiotic Hydrothermal Vent Tubeworm Ridgeia piscesae

摘要: The relationships between hydrothermal vent tubeworms and sulfide-oxidizing bacteria have served as model associations for understanding chemoautotrophy endosymbiosis. Numerous studies focused on the physiological biochemical adaptations that enable these symbioses to sustain some of highest recorded carbon fixation rates ever measured. However, far fewer explored molecular mechanisms underlying regulation host symbiont interactions, specifically those mediated by innate immune system host. To end, we conducted a series where maintained tubeworm, Ridgeia piscesae, in high-pressure aquaria examined global quantitative changes gene expression via high-throughput transcriptomics real-time PCR (qPCR). We analyzed over 32,000 full-length expressed sequence tags well 26 Mb transcript sequences from trophosome (the organ houses endosymbiotic bacteria) plume gas exchange contact with free-living microbial community). R. piscesae under conditions promote number putative cell signaling immunity genes, including pattern recognition receptors (PRRs), often associated recognizing microbe-associated patterns (MAMPs). Eighteen genes involved immunity, signaling, stress metabolite were further using qPCR. PRRs, five peptidoglycan proteins Toll-like receptor, significantly higher compared plume. Although PRRs are mediating responses infection pathogens, differences also implicate similar interactions symbiont. posit this association involves “dialogue” partners includes host’s