Electrokinetic device design and constraints for use in high conductance solutions

摘要: The quest for new cell-free DNA and exosome biomarker-based molecular diagnostics require fast efficient sample preparation techniques. Conventional methods isolating these biomarkers from blood are both time-consuming laborious. New electrokinetic microarray devices using dielectrophoresis (DEP) to isolate have now greatly improved the process. Nevertheless, still some limitations when used with high conductance biological fluids, e.g. blood, plasma, serum. This study demonstrates that electrochemical damage may occur on platinum electrodes of DEP devices. It further examines two model device designs include a parallel wire arrangement planar array. Effective isolation fluorescent beads wires is shown under low-conductance conditions (10−4 S/m), but electrothermal flow overcomes forces (>0.1 S/m). Planar be effective (∼1 S/m) without deleterious effects flow. provides insights into design compromises producing future better performance solutions. This article protected by copyright. All rights reserved