Defined medium supporting development of cleansed Plasmodium berghei ookinetes in Anopheles stephensi

摘要: Abstract Hamsters blood infected with Plasmodium berghei was cultured in vitro for the development of ookinetes. The ookinetes were separated from components, suspended various defined media and fed to Anopheles stephensi through a membrane. oocysts infective sporozoites recorded. Mosquitoes i L15 formulated into L15-B, L15-D (a medium specially modified this purpose), IPL-41 or 199 no proteins added, developed at least as many control mosquitoes blood. Ookinetes L15-B yielded more than after feeding 5% casein. Sporozoites maintained on blood, L15-D, casein shown be hamsters. sucrose solutions showed very few oocysts, but yield increased when meal given 2–4 days meal. Some which had artificial found have degenerated. described system could potentially useful study interaction between vector physiology parasite. possible use learn should future cultivation is discussed.