Induction of phenotypic differentiation, interleukin 2 production, and PHA responsiveness of "immature" human thymocytes by interleukin 1 and phorbol ester.

摘要: Small human thymocytes (ST) representing 70% of the were isolated according to size by centrifugal elutriation. Although these ST contained approximately 30% PNA-cells, they failed respond lectins, indicating existence a PNA-ST subset that can be considered belong "immature" thymocyte population. The induced proliferate if, in addition PHA, IL 1-containing supernatants highly purified monocyte cultures or 12-O-tetradecanoyl-phorbol-13-acetate (TPA) present. incubation for 90 hr with TPA 1 absence PHA resulted strong reduction percentage cells reacting immature markers TdT and PNA. In addition, OKT6+ partially reduced after 1. Concomitantly, an increase mature T cell OKT1 OKT3 was observed, whereas HLA antigens became strongly expressed on all ST. unable induce proliferation ST, substances 2 production cells. These shifts more "mature" phenotypes are able produce not observed if incubated culture medium only. responder capacity plus significantly affected depletion OKT3+ OKT1+ this situation OKT1+, OKT3+, OKT6- found generated from OKT1-, OKT3-, Therefore, it could excluded proliferative responses due selective expansion preexisting Our results indicate mimics induction differentiation stage which subpopulations PHA. Because only proliferating react monoclonal antibody, is thought directed at receptor (anti-Tac), our data suggest required expression receptors those presence situ.