作者: Vahid Khalaj , Parisa Azerang , Shoeib Moradi , Soroush Sardari
DOI:
关键词: Aspergillus niger 、 Plant defensin 、 In silico 、 Peptidomimetic 、 Antimicrobial 、 Antimicrobial peptides 、 Candida albicans 、 Biology 、 Microbiology 、 Biochemistry 、 Aspergillus fumigatus
摘要: Background: The rise of opportunistic fungal infections highlights the need for development new antimicrobial agents. Antimicrobial Peptides (AMPs) and Antifungal (AFPs) are among agents with minimal resistance being developed against them, therefore they can be used as structural templates design Methods: In present study four antifungal peptidomimetic structures named C1 to C4 were designed based on plant defensin Pisum sativum. Minimum inhibitory concentrations (MICs) these determined Aspergillus niger N402, Candida albicans ATCC 10231, Saccharomyces cerevisiae PTCC 5052. Results: C2 showed more potent activity strains compared C3 C4. structure demonstrated a them could template future peptidomemetics design. Sequences alignments led identifying decapeptide (KTCENLADTY) KTC-Y, which its MIC was protoplast showing 25 (μg/ml) fumigatus Af293. Conclusion: approach reach molecules seems powerful in bioactive AMP mimetic identification