Antifungal Indole and Pyrrolidine-2,4-Dione Derivative Peptidomimetic Lead Design Based on In Silico Study of Bioactive Peptide Families

作者: Vahid Khalaj , Parisa Azerang , Shoeib Moradi , Soroush Sardari

DOI:

关键词: Aspergillus nigerPlant defensinIn silicoPeptidomimeticAntimicrobialAntimicrobial peptidesCandida albicansBiologyMicrobiologyBiochemistryAspergillus fumigatus

摘要: Background: The rise of opportunistic fungal infections highlights the need for development new antimicrobial agents. Antimicrobial Peptides (AMPs) and Antifungal (AFPs) are among agents with minimal resistance being developed against them, therefore they can be used as structural templates design Methods: In present study four antifungal peptidomimetic structures named C1 to C4 were designed based on plant defensin Pisum sativum. Minimum inhibitory concentrations (MICs) these determined Aspergillus niger N402, Candida albicans ATCC 10231, Saccharomyces cerevisiae PTCC 5052. Results: C2 showed more potent activity strains compared C3 C4. structure demonstrated a them could template future peptidomemetics design. Sequences alignments led identifying decapeptide (KTCENLADTY) KTC-Y, which its MIC was protoplast showing 25 (μg/ml) fumigatus Af293. Conclusion: approach reach molecules seems powerful in bioactive AMP mimetic identification

参考文章(74)
Victor Lorian, Antibiotics in laboratory medicine Published in <b>2005</b> in Philadelphia by Lippincott Williams & Wilkins. ,(2005)
Karola Rück-Braun, Peter Henklein, Melanie Füllbeck, Ines Stephanie Jaeger, Elke Michalsky, Hartmut Kuhn, Robert Preissner, Design and biological evaluation of photo-switchable inhibitors. Genome Informatics. ,vol. 17, pp. 141- 151 ,(2006) , 10.11234/GI1990.17.141
Michael S Rosenberg, Multiple sequence alignment accuracy and evolutionary distance estimation. BMC Bioinformatics. ,vol. 6, pp. 278- 278 ,(2005) , 10.1186/1471-2105-6-278
Andrean Goede, Elke Michalsky, Ulrike Schmidt, Robert Preissner, SuperMimic – Fitting peptide mimetics into protein structures BMC Bioinformatics. ,vol. 7, pp. 11- 11 ,(2006) , 10.1186/1471-2105-7-11
Hexiang Wang, T.B Ng, Qinghong Liu, Alveolarin, a novel antifungal polypeptide from the wild mushroom Polyporus alveolaris Peptides. ,vol. 25, pp. 693- 696 ,(2004) , 10.1016/J.PEPTIDES.2004.01.026
David I. Chan, Elmar J. Prenner, Hans J. Vogel, Tryptophan- and arginine-rich antimicrobial peptides: structures and mechanisms of action. Biochimica et Biophysica Acta. ,vol. 1758, pp. 1184- 1202 ,(2006) , 10.1016/J.BBAMEM.2006.04.006
Yong Xie, Zhen Peng Kai, Stephen S. Tobe, Xi Le Deng, Yun Ling, Xiao Qin Wu, Juan Huang, Li Zhang, Xin Ling Yang, DESIGN, SYNTHESIS AND BIOLOGICAL ACTIVITY OF PEPTIDOMIMETIC ANALOGS OF INSECT ALLATOSTATINS Peptides. ,vol. 32, pp. 581- 586 ,(2011) , 10.1016/J.PEPTIDES.2010.10.016