Cancer biomarkers in human atherosclerotic lesions: detection of DNA adducts.

摘要: Since somatic mutations are suspected to contribute the pathogenesis not only of cancer but also atherosclerotic plaques, we measured DNA adducts in smooth muscle layer lesions abdominal aorta specimens taken at surgery from seven patients. were evaluated three laboratories by means different molecular dosimetry methods, including: (a) HPLC/fluorescence, which specifically identifies anti-benzo(a)pyrene (BPDE) isomer; (b) two- and three-dimensional synchronous fluorescence spectrophotometries, detect BPDE other reactive metabolites polycyclic aromatic hydrocarbons; (c) 32P postlabeling, reveals presence a variety types adducts. The HPLC/fluorescence method provided for first time evidence BPDE-DNA specific six tested. Synchronous spectrophotometry displayed broad areas all specimens, thereby suggesting occurrence BDPE-DNA with similar characteristics. All positive revealed multiple spots detectable following enrichment either nuclease P1 or butanol, indicative Thus, data obtained applying typical biomarkers provide further support hypothesis that there may be similarities between carcinogenic atherogenic processes, particular genetic alterations caused DNA-binding agents artery wall detected lesions.