Characterizing differences in the collagen fiber organization of skin wounds using quantitative polarized light imaging.

摘要: Collagen fiber organization requires characterization in many biomedical applications, but it is difficult to objectively quantify standard histology tissue sections. Quantitative polarized light imaging a low-cost technique that allows for rapid measurement of collagen orientation and thickness. In this study, we utilize quantitative system characterize thickness from wound Full skin sections were previously stained with hematoxylin eosin used assess content at different points during the healing process. Overall, wounds exhibited measurable increase nonlinear change reorganization within wound. Our study demonstrates an inexpensive alternative or supplement protocols, requiring no additional stains dyes, yields repeatable assessments organization.