Surface modifications and cell-materials interactions with anodized Ti.

摘要: The objective of this study was to investigate in vitro cell–materials interactions using human osteoblast cells on anodized titanium. Titanium is a bioinert material and therefore becomes encapsulated after implantation into the living body by fibrous tissue that isolates it from surrounding tissues. In work, bioactive TiO2 layer grown commercially pure titanium substrate an anodization process different electrolyte solutions, namely H3PO4, HF H2SO4. These electrolytes produced films with nonporous structure showing three distinctive surface morphologies. Human cell growth behavior studied as-received surfaces osteoprecursor line (OPC 1) for 3, 5 11 days. When were compared interaction, noticed each has properties, which led variations interactions. Colonization cell-to-cell attachment surface. Good cellular adherence extracellular matrix extensions between samples H3PO4 electrolyte. H2SO4 did not show significant surface, some death also noticed. Cell adhesions differentiation more pronounced vinculin protein alkaline phosphatase, respectively, surfaces. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assays showed increase density proliferation It clear rough morphology, high energy low values contact angles important factors better materials interaction. A mineralization done simulated fluid ion concentrations nearly identical those blood plasma further understand biomimetic apatite deposition behavior. Similar mineral electrolytes.