Isolation of radiosensitive and radioresistant mutants from a medulloblastoma cell line.
作者： Koji Tsuboi , Yukihiro Tsuchida , Kiyoshi Endo , Yoshihiko Yoshii , Tadao Nose
DOI: 10.1007/BF02478864
关键词: Genetics 、 Radioresistance 、 Mutant 、 DNA damage 、 Cell killing 、 Biology 、 Clone (cell biology) 、 Pathology 、 Medulloblastoma 、 Molecular biology 、 Cell culture 、 Radiosensitivity
摘要: Two mutant clones, one radiosensitive (OS-3) and resistant (OR-5), were isolated from ONS-76 after screening 2400 clones by the replica micro-well technique. These two exhibited significantly different radiosensitivity, with D37 values of 4.7 Gy in OR-5 1.7 OS-3. After gamma irradiation (8 Gy), greater G2 arrest than sensitive clone Administration 5 mM caffeine resulted cell killing OS-3, an almost complete release block. observations support notion that block contributes to repair process DNA damage irradiation. The present results suggest a large postirradiation may show reduction radiosensitivity if is released artificially. study described herein provide important clues mechanism which glioma cells acquire radioresistance.
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