Morphological analysis of ghrelin and its receptor distribution in the rat pancreas.

摘要: Ghrelin, a novel peptide isolated from stomach tissue of rats and humans, has been identified as the endogenous ligand for growth hormone secretagogue receptor (GHS-R). In addition to its secretion stomach, ghrelin is also expressed in hypothalamic arcuate nucleus, intestine, kidney, placenta, pancreas. GHS-R mRNA, on other hand, hypothalamus, pituitary, heart, lung, liver, pancreas, adipose tissue. Ghrelin considered have important roles feeding regulation energy metabolism well release (GH). Recent physiological experiments pancreas shown that regulates insulin secretion. However, sites action are yet be identified. this study, gain insight into role rat pancreatic islets, we used immunohistochemistry determine localization islet cells. Double fluorescence revealed weak GHS-R-like immunoreactivity was found B cells containing insulin. overlapped glucagon-like immunoreactive Moreover, both immunoreactivities were detected mostly same periphery islets Langerhans. These observations suggest synthesized secreted A cells, acts back an autocrine and/or paracrine manner. addition, may act via regulate