Long term shear stress leads to increased phosphorylation of multiple MAPK species in cultured human aortic endothelial cells.

摘要: Fluid dynamics strongly influences endothelial cell function, and participates in the localization of atherosclerotic plaques at blood vessel branches. We investigated hypothesis that wild-type human aortic cells (HAEC) exposed to prolonged pulsatile flow stimulation have levels phosphorylated mitogen-activated protein kinases (MAPK) are significantly greater than those observed statically grown cultures. HAEC were laminar shear stress a parallel-plate chamber analyzed for ERK, JNK p38 1, 10 20 h. While some MAPK exhibited alternating patterns phosphorylation, others characterized by steady increases or unchanged profiles until terminal (20 h) time point. However, h, each demonstrated an increase phosphorylation versus cultivated cells. Further, h cultures from dyn/cm(2) had higher 2 dyn/cm(2). The finding species can be response both magnitude dependent manner is interesting result may help explain how differential behaviors between different environments generated maintained.