Programmed ribosomal frameshifting in decoding the SARS-CoV genome
作者： Pavel V. Baranov , Clark M. Henderson , Christine B. Anderson , Raymond F. Gesteland , John F. Atkins
DOI: 10.1016/J.VIROL.2004.11.038
关键词: Frameshift mutation 、 Pseudoknot 、 Biology 、 Ribosomal frameshift 、 Nucleic acid structure 、 Transfer RNA 、 RNA 、 Genetics 、 Nucleic acid secondary structure 、 Translational frameshift 、 Virology
摘要: Programmed ribosomal frameshifting is an essential mechanism used for the expression of orf1b in coronaviruses. Comparative analysis frameshift region reveals a universal shift site U_UUA_AAC, followed by predicted downstream RNA structure form either pseudoknot or kissing stem loops. Frameshifting SARS-CoV has been characterized cultured mammalian cells using dual luciferase reporter system and mass spectrometry. Mutagenic spectrometry affinity tagged product confirmed tandem tRNA slippage on sequence U_UUA_AAC. Analysis stimulator shows that proposed secondary loop II two unpaired nucleotides at I-stem junction are important stimulation. These results demonstrate key sequences required efficient frameshifting, utility to study frameshifting.
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