A novel method for single sample multi-axial nanoindentation of hydrated heterogeneous tissues based on testing great white shark jaws.

摘要: Nanomechanical testing methods that are suitable for a range of hydrated tissues crucial understanding biological systems. Nanoindentation can provide valuable insights into biology, tissue engineering and biomimetic design. However, samples still remains significant challenge. Shark jaw cartilage is an ideal substrate developing method to test because it unique heterogeneous composite both mineralized (hard) non-mineralized (soft) layers possesses geometry challenging mechanically. The aim this study develop novel obtaining multidirectional nanomechanical properties from single sample, taken the great white shark (Carcharodon carcharias). A data sample necessary examining mechanics in protected species hence may be difficult obtain. Results show maintains hydration would otherwise rapidly dehydrate. Our first analysis cartilage. Variation were detected different orthogonal directions species. further suggest layer less stiff than previously posited. allows obtained single, small, sample. technique therefore use when specimens rare, or limited quantity, such as endangered pathological tissues. We also outline tip-to-optic calibration facilitates nanoindentation soft help address critical need applicable variety materials whether hard.