Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures.

摘要: Tissue engineering focuses on the repair and regeneration of tissues through use biodegradable scaffold systems that structurally support regions injury whilst recruiting and/or stimulating cell populations to rebuild target tissue. Within bone tissue engineering, effects architecture cellular response have not been conclusively characterized in a controlled-density environment. We present theoretical practical assessment polycaprolactone (PCL) architectural modifications mechanical flow characteristics as well MC3T3-E1 preosteoblast an vitro static plate custom-designed perfusion bioreactor model. Four architectures were contrasted, which varied inter-layer lay-down angle offset between layers, maintaining structural porosity 60 ± 5%. established layer was decreased (90° vs. 60°) introduced (0 0.5 layers), stiffness, yield stress, strength, pore size permeability decreased, computational fluid dynamics-modeled wall shear stress increased. Most significant noted with offset. Seeding efficiencies culture also dramatically increased due (~45% ~86%), exhibiting much higher seeding efficiency than culture. Scaffold had minimal effect However, influenced osteogenic differentiation culture, likely by modifying microfluidic