Compositions and methods for detecting small rnas, and uses thereof

作者: Mark Alan Reynolds , Amara Siva

DOI:

关键词: Reverse primerTarget captureSmall RNADouble strandedBiologyMirna targetCombinatorial chemistryPrimer (molecular biology)Nucleic acidMolecular biologyOligomer

摘要: Compositions and methods are provided for the detection of small RNA target nucleic acids, preferably miRNA wherein compositions provide sensitive specific acids. The include using one or more a first amplification oligomer that is an extender primer, capture at least partially double stranded, promoter primer/provider, reverse primer universal probe. useful diagnostics, prognostics, monitoring effectiveness treatment and/or determining treatment.

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