Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide

摘要: A method for determining the presence and amount of a predetermined target nucleotide sequence in biological sample is provided. The employs reagent complex which consists labeled probe polynucleotide having binding region second bound to thereof. at least partially coextensive with region. This contacted under conditions binds probe, displacing polynucleotide. Labeled probe/target hybrids are separated from intact complexes these determined.