Method for purification and identification of sperm cells
作者： Glenn Sanders , Larry Gold , Evaldas Katilius
DOI:
关键词: Sperm 、 Sperm cell 、 Molecular biology 、 Biology 、 HeLa 、 Biochemistry 、 Semen 、 DNA 、 Aptamer
摘要: The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity specificity to capture immobilize intact sperm cells in presence female epithelial other non-sperm semen components. also affinity-based methods for detection samples, including from forensic sample surrogates consisting swab eluates containing a mixture HeLa cells. eluting cell samples swabs; purifying amplification analysis male DNA. system described herein is inexpensive, simple use easily implemented laboratories.
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