Orofacial infection of athymic mice with defined mixtures of acyclovir-susceptible and acyclovir-resistant herpes simplex virus type 1

摘要: Infection of athymic mice with defined populations acyclovir-susceptible (thymidine kinase [TK]-positive) and acyclovir-resistant (TK-deficient or TK-altered) herpes simplex virus type 1 strains was used to simulate herpetic skin disease the immunocompromised host. In vitro characterization mixtures revealed that dye uptake method quite sensitive in detection small amounts (3 9%) acylovir-resistant virus. Mice infected homogeneous exhibited a good correlation between clinical response drug susceptibility infecting Animals viruses varied patterns infection responses acyclovir treatment. However, severity useful predicting TK phenotype recovered from lesions. Pathogenic, TK-altered responsible for progressive animals receiving low-dose (0.25-mg/ml) prophylactic high-dose (1.25-mg/ml) delayed therapy. Although this mutant infrequently, it clinically significant which isolated.