A comparative evaluation of three methods of antiviral susceptibility testing of clinical herpes simplex virus isolates

摘要: Abstract Background: Current methods of antiviral susceptibility testing for herpes simplex virus (HSV) are poorly standardized and have rarely been compared critically. Objectives: To compare the three most commonly utilized HSV assays accuracy result, method implementation, time required. Study design: We results acyclovir foscarnet using plaque reduction, dye uptake DNA hybridization in 30 patient isolates HSV, varying pattern. Compared parameters included: values ID 50 (the concentration drug required to inhibit growth by 50% or more), ratio 90 , correlation result with clinical response therapy, when available. In addition, we ease assay, presence objective endpoint, generate necessary equipment implementation. Results: The yielded that were approximately two-fold greater than those from reduction while assay one-half assay. Comparison therapy 17 instances 10 suggested possibility a somewhat discriminative ability lesser borderline susceptibility. Conclusions: Larger comparative studies further differences HSV. All deemed suboptimal due an overly long turnaround time, associated expense, and/or level their performance. Continued evaluation alternative, more rapid is therefore warranted.