A colorimetric assay for the measurement of the sensitivity of herpes simplex viruses to antiviral agents

摘要: Abstract A quantitative colorimetric method for measuring the inhibition of viral cytopathic effects has been adapted to assay antiviral compounds. Drug-treated, virus-infected cultures in microtiter plates were stained with vital dye neutral red and amount incorporated was determined a multichannel spectrophotometer. The technique required smaller volumes reagents, more easily automated than standard plaque reduction had good reproducibility. Standard conditions 30 infectious units challenge virus 72-h incubation judged be optimal. Median inhibitory concentrations (ID50) number compounds approximately tenfold higher dye-uptake compared assay, possible related multiplicity infection give desired level effect method.