Identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the L component of herpes simplex virus genome.

摘要: In the course of studies on a sequences located at termini and junction between L S components herpes simplex virus 1 DNA, J. Chou B. Roizman (J. Virol. 57:629-637, 1986) noted that sequence acted as gamma promoter when fused to structural thymidine kinase gene, b inverted repeat in component next contained an open reading frame predicted encode protein 358 amino acids with molecular weight 37,054, transcription RNA homologous initiated within sequence. The nucleotide presence triplet Ala-Thr-Pro repeated 10 times. To verify existence designated 134.5, synthetic peptide consisting times was synthesized used raise antibodies rabbits. results were follows. antiserum reacted 43,500-apparent-molecular-weight present lysates cells infected but not mock-infected or 2-infected cells. We genetically engineered recombinant containing single copy truncated gene. Concordant predictions, antibody faster-migrating this recombinant. 134.5 gene product soluble, it accumulated primarily cytoplasm late infection. overlap domain raises possibility acts trans is associated one functions currently ascribed sequences.