Kit and method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells
作者： Chi Jinhuan , Pan Chungang
DOI:
关键词: In vitro 、 Stem cell 、 Cell therapy 、 Cell biology 、 Chemistry 、 Heart development 、 Transplantation 、 Heart Injury 、 Nutrient solution
摘要: The invention provides a kit and method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells. By virtue nutrient solution provided by the disclosed accordance with invention, efficient is promoted. are simple reliable, stable high safety, proportion multipotential significantly increased to be greater than 90%. Meanwhile, culture medium applicable large-scale production high-quality cells, without subsequent screening purifying steps; directly heart development scientific researches, cell therapy diseases, transplantation injury application demand drug screening.
lens.org 参考文章(5)
Yanan Zhu, Songzhu An, Serum-free chemically defined cell culture medium ,(2011)
Andreia Bernardo, Christine Cheung, Roger Pedersen, Sanjay Sinha, Populations of smooth muscle cells of specific embryonic lineages ,(2012)
Fumiyuki Hattori, Keiichi Fukuda, A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses ,(2007)
James Hudson, Wolfram-Hubertus Zimmermann, Malte Tiburcy, A method to direct differentiation of pluripotent stem cells into functional heart muscle ,(2014)
Song Zhihua, Ding Mingxiao, Deng Hongkui, Method for differentiating induced human embryonic stem cells or human induction-formed multipotential stem cells to liver cells ,(2013)