Effect of ammonium on the expression of osmosensitive genes in Madin-Darby canine kidney cells

摘要: The cells of the kidney medulla are exposed routinely to high extracellular concentrations various solutes including NaCl, urea and ammonium (NH4+). Although it is well established that expression a variety osmosensitive genes proteins, which confer cytoprotection on renal medullary cells, induced by NaCl concentrations, role NH4+ in these cellular responses unclear. This study thus addressed effect betaine/GABA transporter (BGT-1), sodium/myo-inositol cotransporter (SMIT), aldose reductase (AR), heat shock protein 70 (HSP70) Madin–Darby canine (MDCK) using Northern Western blot analyses enzyme-linked immunosorbent assay (ELISA). incidence apoptosis was monitored determining caspase-3 activity annexin V binding. Addition NH4Cl (50 mm; total osmolality 400 mosmol (kg H2O)−1 medium more effective than equiosmolar increasing BGT-1 HSP70 mRNA abundance, but less enhancing at level. Qualitatively similar results were obtained for SMIT AR mRNAs. Exposure both isotonic hypertonic, NH4Cl-containing enhanced compared with equiosmolar, NaCl-containing media. These suggest that, addition may play regulating intracellular accumulation organic osmolytes, abundance cell turnover vivo.