Proasthmatic effects and mechanisms of action of the dust mite allergen, Der p 1, in airway smooth muscle.

摘要: Background House dust mite allergen exposure is a key risk factor for the development of allergic asthma. Beyond provoking immune cell-mediated responses, house allergens were recently shown to exert direct effects on airway structural cells secondary their intrinsic protease activities. Objective This study tested hypothesis that can produce changes in responsiveness through effect smooth muscle (ASM). Methods Isolated rabbit ASM tissues exposed allergen, Der p 1, and induced activation mitogen-activated protein kinase (MAPK) signaling pathways examined under different experimental conditions. Results The observations demonstrated following: (1) 1 elicited enhanced constrictor responses impaired relaxation tissues, (2) these proasthmatic-like attributed its cysteine activity, (3) associated with both extracellular signal-regulated (ERK) 1/2 p38 MAPK pathways. Additionally, specific blockade ERK1/2 was found prevent 1–induced responsiveness, whereas inhibition action latter result augmented ERK1/2. Conclusion These findings are first demonstrate directly elicit signaling, wherein proasthmatic by ERK1/2, coactivation exerts homeostatic negatively regulating signaling.