Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production.

摘要: Abstract Background: Proteases in extracts of Aspergillus fumigatus cause epithelial cell desquamation and release proinflammatory cytokines. Objective: We sought to assess protease activity Alternaria alternata , Cladosporium herbarum study the ability these cytokines from cells. Methods: Protease activities fungal were quantified. Changes with respect morphology, desquamation, cytokine production (IL-6 IL-8) measured absence presence an airway-derived line (A549) primary nasal Results: Fungal proteases differentially induced morphologic changes, IL-6 IL-8 a dose- time-dependent fashion. shrinking strongly enhanced at higher concentrations. caused shrinking, IL-8, even low The –derived extract grown on collagen medium strong dose-dependent decline did not induce changes or but productions dependence effects intact was shown by their abrogation inhibitors. Conclusion: present interact cells, leading induction It is proposed that may activate cells through protease-activated receptor type 2–driven mechanism. (J Allergy Clin Immunol 2000;105:1185-93.)