The enigma of progressively partial endoreplication: new insights provided by flow cytometry and next generation sequencing

摘要: In many plant species, somatic cell differentiation is accompanied by endoreduplication, a process during which cells undergo one or more rounds of DNA replication cycles in the absence mitosis, resulting nuclei with multiples 2C amounts (4C, 8C, 16C, etc.). some orchids, disproportionate increase nuclear contents has been observed, where successive endoreduplication result + P, 3P, 7P, etc., P content replicated part genome. This unique phenomenon was termed "progressively partial endoreplication" (PPE). We investigated processes behind PPE Ludisia discolor using flow cytometry (FCM) and Illumina sequencing. particular, we wanted to determine whether chromatin elimination incomplete genome duplication involved, identify types sequences that were affected. Cell cycle analysis root tip pulse-labeled EdU revealed two cycles, ending above population content, other typical "horseshoe" pattern S-phase ranging from 4C contents. The leading therefore involves replication. Subsequent sequencing flow-sorted showed all repetitive affected PPE; complete any specific type not observed. hypothesize highly controlled transition mechanism proliferation phase tissue development.