Flow cytometric seed screen data are consistent with models of chromosome inheritance in asymmetrically compensating allopolyploids

摘要: Angiosperms have evolved a mechanism of double fertilization, which results in the production separate embryo (new individual) and endosperm (nutritive tissue). The flow cytometric seed screen (FCSS) was developed to infer plant reproduction modes based on endosperm-to-embryo DNA content ratio (Pind ). A 1.5 indicates sexual reproduction, whereas higher values ≥2.0 are consistent with apomixis. Although FCSS has been successfully applied study asexual plants, limits particularly its potential for determination hemisexual plants not explored. Here, we evaluated application two asymmetrically compensating allopolyploids (ACAs), Onosma arenaria Rosa canina. These species characterized by presence asexually inherited univalent-forming sexually bivalent-forming chromosome sets. They both use asymmetric meiosis, eliminates sets from male gamete retains them female gamete. Different chromosomal behavior meiosis these is reflected different theoretically derived Pind values, deviate value. determined FCSS-based seeds ACAs, compared species. As expected, that mean 1.51, 1.52, 1.52 is, Capsella bursa-pastoris, Crataegus monogyna, O. pseudoarenaria, respectively. In were (1.61) R. canina (1.82). theoretical models inheritance. This highlights precision cytometry determining it's utility screening modes. Additionally, it advocates more in-depth investigations into rapid accessions where deviated value typical species, may indicate meiotic irregularities.