A model for the biogenesis of Turnip mosaic virus replication factories

摘要: Nicotiana benthamiana plants were agroinfiltrated with an infectious clone of the Turnip mosaic virus (TuMV) that was engineered to tag replication vesicles either GFP or mCherry fluorescent proteins. Punctuate vesicle structures observed in cytoplasm infected cells corresponding viral factories. The highly motile and co-aligned microfilaments. Utilization latrunculin B, inhibitor microfilament polymerization, reduced accumulation virus, suggesting microfilaments are necessary during infection. To investigate biogenesis vesicles, leaves simultaneously two recombinant TuMV clones, one labeled red them green. We cell green only indicating a single genome origin. In some cases, exhibited sectors green, yellow fluorescence also observed, demonstrating fusion among individual is possible. Based on those results we propose model for factory, where translation tightly coupled within virus-induced vesicles.