The IRE1/bZIP60 Pathway and Bax Inhibitor 1 Suppress Systemic Accumulation of Potyviruses and Potexviruses in Arabidopsis and Nicotiana benthamiana Plants.

摘要: The inositol requiring enzyme (IRE1) is an endoplasmic reticulum (ER) stress sensor. When activated, it splices the bZIP60 mRNA, producing a truncated transcription factor that upregulates genes involved in unfolded protein response. Bax inhibitor 1 (BI-1) another ER sensor regulates cell death response to environmental assaults. potyvirus 6K2 and potexvirus TGB3 proteins are known reside ER, serving, respectively, as anchors for viral replicase movement complex. This study used green fluorescent (GFP)-tagged Turnip mosaic virus (TuMV), Plantago asiatica (PlAMV), Potato Y (PVY), X (PVX) determine IRE1/bZIP60 pathway BI-1 machinery induced early infection Arabidopsis thaliana, Nicotiana benthamiana, Solanum tuberosum. Agrodelivery of only or into plant cells activated expression N. S. Homozygous ire1a-2, ire1b-4, ire1a-2/ire1b-4 mutant plants were inoculated with TuMV-GFP PlAMV-GFP. PlAMV accumulates higher level ire1a-2 than ire1b-4 wild-type plants. compared plants, suggesting both isoforms contribute infection. Gene silencing was knock down benthamiana. PVX-GFP PVY-GFP accumulation significantly elevated these silenced control demonstrates two pathways, namely pathway, limit systemic Silencing also resulted necrosis. These data suggest stress-activated led by IRE1 BI-1, respond invading potexviruses restrict enable physiological changes enabling tolerate assault.