Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana

摘要: Endoplasmic reticular (ER) membrane modifications play an important role in viral RNA replication and virion assembly but little is known about the involvement of ER-membrane remodeling infection cycle fijiviruses plant cells. The subcellular localization Rice black-streaked dwarf virus outer capsid P10 was therefore examined using live-cell imaging. fused to eGFP formed vesicular structures associated with ER membranes Nicotiana benthamiana epidermal cells rice protoplasts. Subcellular fractionation experiments confirmed that integral protein. Three predicted transmembrane domains two less-well-defined were each able target ER. Disruption actin cytoskeleton LatB, indicated maintenance P10-induced required intact cytoskeleton. induced expression stress marker genes, including stress-related chaperones transcription factor, indicating RBSDV triggers unfolded protein response.