Effects of nicotine on cultured rat heart cells

摘要: Abstract Cells from hearts of 2- to 4-day-old rats were separated into cultures 93% endothelioid (E) cells, and 95% muscle (M) on the basis rapid attachment E-cells culture dishes. beat longer in composed chiefly M-cells than unseparated cultures. Incorporation nicotine alkaloid (0.003 – 0.6 m ) medium M-cell produced a further increase duration beating slowed time-related decline rate. These actions not attributable effects cell or stimulation cells which had stopped beating. Differences between two types response higher concentrations (1.5 6.0 ), demonstrated by vacuole formation nuclear changes. Untreated cultures, initially either M-cells, differed glucose-6-phosphate dehydrogenase (G-6-PDH), lactic (LDH) its isoenzymes, pyruvic kinase (PK) isocitric linked nicotinamide adenine dinucleotide phosphate (ICDH-NADP) activities over an 18-day period. With exception decreased fall LDH-H subunit activity both types, treatment with (0.016 did alter any enzyme measured.