A model for random genetic damage directing selection of diploid or aneuploid tumours

摘要: Objectives:  To test whether genetic instability may determine tumours become aneuploid or diploid. Materials and methods:  We have identified genes needed for cell survival replication by combining Affymetrix gene expression array data from 12 experimental lines with in silico GEO+GNF expO databases. Specific loss of heterozygosis (LOHs), chromosomal abnormalities (called derivative chromosomes) numbers normal homologues were SNP SKY analyses. Random losses calculated under the assumption that bi-allelic MMR inactivation causes a 20-fold increase rate loss. Results:  There ∼1.23 × 104 widely dispersed throughout genome possibly expressed all cells proliferation, many these performed housekeeping functions. Conservation explain complete haploid genomes found 15 different types chromosomes selectively retained cancer after LOH formations, homologue losses. Loss survival/replication was to be higher colon stem carriers mutations than APC mutations. Conclusion:  low enough ‘select’ chromosome combinations favouring tumour proliferation. However, too high colonic lacking survive instability, explaining why only produce diploid cells.