Identification of aneuploidy-tolerating mutations
作者： Eduardo M. Torres , Noah Dephoure , Amudha Panneerselvam , Cheryl M. Tucker , Charles A. Whittaker
DOI: 10.1016/J.CELL.2010.08.038
关键词: Saccharomyces cerevisiae 、 Mutation 、 Aneuploidy 、 Gene 、 Cancer cell 、 Cancer 、 Biology 、 Cell growth 、 Deubiquitinating enzyme 、 Genetics
摘要: Aneuploidy causes a proliferative disadvantage in all normal cells analyzed to date, yet this condition is associated with disease characterized by unabated potential, cancer. The mechanisms that allow cancer tolerate the adverse effects of aneuploidy are not known. To probe question, we identified aneuploid yeast strains improved abilities. Their molecular characterization revealed strain-specific genetic alterations as well mutations shared between different strains. Among latter, loss-of-function mutation gene encoding deubiquitinating enzyme Ubp6 improves growth rates four attenuating changes intracellular protein composition caused aneuploidy. Our results demonstrate existence aneuploidy-tolerating improve fitness multiple aneuploidies and highlight importance ubiquitin-proteasomal degradation suppressing
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