Detection of genetic variation in Bradyrhizobium japonicum USDA 110 variants using DNA fingerprints generated with GC rich arbitrary PCR primers
作者： James N. Mathis , David E. McMillin
DOI: 10.1007/978-94-011-5700-1_11
关键词: Enzyme 、 Bradyrhizobium japonicum 、 Genetic marker 、 Genetics 、 Homology (biology) 、 Genetic variation 、 Nitrogen fixation 、 DNA profiling 、 DNA 、 Biology
摘要: Bradyrhizobium japonicum USDA 110 has been shown to contain several genetically similar naturally occurring colony morphology variants. These variants differ in symbiotic nitrogen fixation ability and the utilization of various carbon substrates. They have share extensive DNA homology appear be derived from a common ancestor. Despite these similarities certain B. devoid fixation. One (L2-110), however, was recently possess significant levels ex planta synthesize functional dinitrogenase enzyme within bacteroids. In an effort identify genetic markers which could explain differences between variants, fingerprints were generated by PCR using arbitrary primers. Two primers with GC rich sequences able differentiate 1-110, L2-110, MN-110. Unique now identified examined further determine if they
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