Verification of strain identity in Brazilian soybean inoculants by using the Polymerase Chain Reaction
作者： Marilu L. Sato , Cayo García-Blásquez , Peter van Berkum
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摘要: The standards for meeting inoculant quality in Brazil include a requirement of strain verification as well the necessity minimal number viable bacterial cells. This stimulated us to develop rapid and simple PCR-based method distinguish four Brazilian strains Bradyrhizobium, SEMIA 587, 5019 (29W), 5079 (CPAC-15) 5080 (CPAC-7), recommended inoculation soybean. PCR reactions using random amplified polymorphic DNA (RAPD) primer CRL-7 together with modified buffer resulted different fingerprint patterns, which permitted soybean be distinguished from each other. patterns obtained were reproducible irrespective source templates. Comparisons bradyrhizobia USDA Culture Collection led conclusion that two (SEMIA 587 5019) characteristic B. elkanii, while other 5080) more typical japonicum. Also, we evidence RAPD analysis may useful distinguishing variants same strain.
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